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PANK2 Polyclonal antibody

PANK2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11001-1-AP

Print datasheet

Synonyms

Pantothenate kinase 2, mitochondrial intermediate form, mPanK2, iPanK2, hPanK2, EC:2.7.1.33

验证数据展示

  • WB analysis using 11001-1-AP

    WB analysis using 11001-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 11001-1-AP

    IP experiment of mouse brain using 11001-1-AP

    IP result of anti-PANK2 (IP:11001-1-AP, 3ug; Detection:11001-1-AP 1:800) with mouse brain tissue lysate 9500ug.

  • IHC staining of human lung cancer using 11001-1-AP

    IHC staining of human lung cancer using 11001-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11001-1-AP (PANK2 antibody) at dilution of 1:10 (under 10x lens).

  • IF Staining of HepG2 using 11001-1-AP

    IF Staining of HepG2 using 11001-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PANK2 antibody (11001-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inK-562 cells, human brain tissue, MCF-7 cells, mouse brain tissue, mouse testis tissue, rat brain tissue, rat testis tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lung cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:10-1:100
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11001-1-AP targets PANK2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PANK2 fusion protein Ag1413 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pantothenate kinase 2
Synonyms Pantothenate kinase 2, mitochondrial intermediate form, mPanK2, iPanK2, hPanK2, EC:2.7.1.33
Calculated Molecular Weight 63 kDa
Observed Molecular Weight63-70 kDa
GenBank Accession NumberBC009421
Gene Symbol PANK2
Gene ID (NCBI) 80025
RRIDAB_2159041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZ23
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pantothenate kinase 2 (PANK2) is an essential regulatory enzyme in coenzyme A biosynthesis. PANK2 mutations cause pantothenate kinase-associated neurodegeneration (PKAN), which leads to pigmentary retinopathy, progressive dystonia and other abnormalities(PMID:20603201). PanK2 protein is localized to mitochondria of neurons in human brain, distinguishing it from other pantothenate kinases that do not possess mitochondrial-targeting sequences(PMID:15659606 ). It has 4 isoforms produced by alternative splicing and alternative initiation.

实验方案

Product Specific Protocols
WB protocol for PANK2 antibody 11001-1-APDownload protocol
IHC protocol for PANK2 antibody 11001-1-APDownload protocol
IF protocol for PANK2 antibody 11001-1-APDownload protocol
IP protocol for PANK2 antibody 11001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols