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验证数据展示

  • WB analysis using 18095-1-AP

    WB analysis using 18095-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 18095-1-AP (PAPPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 18095-1-AP

    IHC staining of human placenta using 18095-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18095-1-AP (PAPPA antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAPPA antibody included

PAPPA Polyclonal antibody

PAPPA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18095-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

ASBABP2, DIPLA1, IGF dependent IGFBP 4 protease, IGFBP 4ase, PAPA, PAPP A, PAPPA, PAPPA1, Pappalysin 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PAPPA Ready-To-Use IHC Kit. PAPPA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18095-1-AP targets PAPPA in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 PAPPA fusion protein Ag11863 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pregnancy-associated plasma protein A, pappalysin 1
别名 ASBABP2, DIPLA1, IGF dependent IGFBP 4 protease, IGFBP 4ase, PAPA, PAPP A, PAPPA, PAPPA1, Pappalysin 1
计算分子量 1627 aa, 181 kDa
观测分子量180-220 kDa
GenBank蛋白编号BC078657
基因名称 PAPPA
Gene ID (NCBI) 5069
RRIDAB_3085554
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13219
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pregnancy-associated plasma protein A (PAPPA) is a metalloproteinase secreted by human placenta, and it can interact with IGF-binding proteins (IGFBPs) to regulate the proteolysis and the bioavailability of insulin-like growth factor (IGF). Circulating PAPPA represents a consolidated biomarker in combined first-trimester screening tests, allowing an early prenatal diagnosis of chromosomal abnormalities, preeclampsia, and intrauterine growth restriction risk. PAPPA is highly expressed in placenta and pregnancy serum (PMID: 34974815, 34974815).

实验方案

Product Specific Protocols
WB protocol for PAPPA antibody 18095-1-APDownload protocol
IHC protocol for PAPPA antibody 18095-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols