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验证数据展示

  • WB analysis of human placenta using 66962-1-Ig

    WB analysis of human placenta using 66962-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66962-1-Ig (PAPPA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 66962-1-Ig

    WB analysis of MCF-7 using 66962-1-Ig

    WB result of PAPPA antibody (66962-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAPPA transfected MCF-7 cells.

  • IHC staining of human placenta using 66962-1-Ig

    IHC staining of human placenta using 66962-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66962-1-Ig (PAPPA antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAPPA antibody included
  • IHC staining of human placenta using 66962-1-Ig

    IHC staining of human placenta using 66962-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66962-1-Ig (PAPPA antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAPPA antibody included
  • Featured Product
  • KD/KO验证

PAPPA Monoclonal antibody

PAPPA Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66962-1-Ig

产品说明书

CloneNo. 1G5A7
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

Human and More (1)

应用

WB, IHC, ELISA

别名

ASBABP2, DIPLA1, IGF dependent IGFBP 4 protease, IGFBP 4ase, PAPA, PAPP A, PAPPA, PAPP-A, PAPPA1, Pappalysin 1, Pappalysin-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, MCF-7 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PAPPA Ready-To-Use IHC Kit. PAPPA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:4000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66962-1-Ig targets PAPPA in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 Human
文献引用反应性human
免疫原 PAPPA fusion protein Ag11863 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 pregnancy-associated plasma protein A, pappalysin 1
别名 ASBABP2, DIPLA1, IGF dependent IGFBP 4 protease, IGFBP 4ase, PAPA, PAPP A, PAPPA, PAPP-A, PAPPA1, Pappalysin 1, Pappalysin-1
计算分子量 1627 aa, 181 kDa
观测分子量 180-220 kDa
GenBank蛋白编号BC078657
基因名称 PAPPA
Gene ID (NCBI) 5069
RRIDAB_2882285
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13219
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pregnancy-associated plasma protein A (PAPPA) is a metalloproteinase secreted by human placenta, and it can interact with IGF-binding proteins (IGFBPs) to regulate the proteolysis and the bioavailability of insulin-like growth factor (IGF). Circulating PAPPA represents a consolidated biomarker in combined first-trimester screening tests, allowing an early prenatal diagnosis of chromosomal abnormalities, preeclampsia, and intrauterine growth restriction risk. PAPPA is highly expressed in placenta and pregnancy serum (PMID: 34974815, 34974815).

实验方案

Product Specific Protocols
WB protocol for PAPPA antibody 66962-1-IgDownload protocol
IHC protocol for PAPPA antibody 66962-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Oncol

Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression.

Authors - Go Itoh
View Article