验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14708-1-AP targets PAPSS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, hamster |
| 免疫原 | PAPSS1 fusion protein Ag6401 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 3'-phosphoadenosine 5'-phosphosulfate synthase 1 |
| 别名 | ATP sulfurylase, APS kinase, Adenylyl-sulfate kinase, Adenylylsulfate 3'-phosphotransferase, Adenosine-5'-phosphosulfate 3'-phosphotransferase |
| 计算分子量 | 71 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC050627 |
| 基因名称 | PAPSS1 |
| Gene ID (NCBI) | 9061 |
| RRID | AB_2159487 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43252 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAPSS1 antibody 14708-1-AP | Download protocol |
| IHC protocol for PAPSS1 antibody 14708-1-AP | Download protocol |
| IF protocol for PAPSS1 antibody 14708-1-AP | Download protocol |
| IP protocol for PAPSS1 antibody 14708-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res CIB2 negatively regulates oncogenic signaling in ovarian cancer via sphingosine kinase 1. | ||
Biotechnol J Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation. | ||
Histol Histopathol Correlation of 3'-Phosphoadenosine-5'-Phosphosulfate synthase 1 (PAPSS1) expression with clinical parameters and prognosis in esophageal squamous carcinoma | ||
bioRxiv Glycosaminoglycan-mediated lipoprotein uptake protects cancer cells from ferroptosis |
