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PARG Monoclonal antibody

PARG Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H10G10

Cat no : 67946-1-Ig

Print datasheet

Synonyms

PARG, PARG99

验证数据展示

  • WB analysis using 67946-1-Ig

    WB analysis using 67946-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67946-1-Ig (PARG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human breast cancer using 67946-1-Ig

    IHC staining of human breast cancer using 67946-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67946-1-Ig (PARG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67946-1-Ig

    IHC staining of human breast cancer using 67946-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67946-1-Ig (PARG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 67946-1-Ig

    IF Staining of A431 using 67946-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PARG antibody (67946-1-Ig, Clone: 1H10G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67946-1-Ig targets PARG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen PARG fusion protein Ag27288 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name poly (ADP-ribose) glycohydrolase
Synonyms PARG, PARG99
Calculated Molecular Weight 111 kDa
Observed Molecular Weight111 kDa
GenBank Accession NumberBC052966
Gene Symbol PARG
Gene ID (NCBI) 8505
RRIDAB_2918698
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86W56
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PARG antibody 67946-1-IgDownload protocol
IHC protocol for PARG antibody 67946-1-IgDownload protocol
IF protocol for PARG antibody 67946-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols