PARG Monoclonal antibody
PARG Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1H10G10
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67946-1-Ig targets PARG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | PARG fusion protein Ag27288 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | poly (ADP-ribose) glycohydrolase |
Synonyms | PARG, PARG99 |
Calculated Molecular Weight | 111 kDa |
Observed Molecular Weight | 111 kDa |
GenBank Accession Number | BC052966 |
Gene Symbol | PARG |
Gene ID (NCBI) | 8505 |
RRID | AB_2918698 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q86W56 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for PARG antibody 67946-1-Ig | Download protocol |
IHC protocol for PARG antibody 67946-1-Ig | Download protocol |
IF protocol for PARG antibody 67946-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |