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  • Featured Product
  • KD/KO Validated

PARK2/Parkin Monoclonal antibody, PBS Only

PARK2/Parkin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H5A7

Cat no : 66674-1-PBS

Print datasheet

Synonyms

Parkin, PARK2, Parkin RBR E3 ubiquitin-protein ligase, EC:2.3.2.31, E3 ubiquitin-protein ligase parkin

验证数据展示

  • WB analysis of HEK-293 using 66674-1-Ig (same clone as 66674-1-PBS)

    WB analysis of HEK-293 using 66674-1-Ig (same clone as 66674-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66674-1-Ig (Parkin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

    IHC staining of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66674-1-Ig (Parkin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PARK2 antibody included
  • IHC staining of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

    IHC staining of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66674-1-Ig (Parkin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PARK2 antibody included
  • IF Staining of RAW 264.7 using 66674-1-Ig (same clone as 66674-1-PBS)

    IF Staining of RAW 264.7 using 66674-1-Ig (same clone as 66674-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using PARK2/Parkin antibody (66674-1-Ig, Clone: 2H5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PARK2 Ready-To-Use IHC Kit. PARK2 primary antibody included.

产品信息

66674-1-PBS targets PARK2/Parkin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen PARK2/Parkin fusion protein Ag5179 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Parkinson disease (autosomal recessive, juvenile) 2, parkin
Synonyms Parkin, PARK2, Parkin RBR E3 ubiquitin-protein ligase, EC:2.3.2.31, E3 ubiquitin-protein ligase parkin
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 42-52 kDa
GenBank Accession NumberBC022014
Gene Symbol Parkin
Gene ID (NCBI) 5071
RRIDAB_2882028
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60260
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing.