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验证数据展示

  • WB analysis of HEK-293 using 66674-1-Ig (same clone as 66674-1-PBS)

    WB analysis of HEK-293 using 66674-1-Ig (same clone as 66674-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66674-1-Ig (Parkin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

    IHC staining of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66674-1-Ig (Parkin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PARK2 antibody included
  • IHC staining of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

    IHC staining of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66674-1-Ig (Parkin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PARK2 antibody included
  • IF Staining of RAW 264.7 using 66674-1-Ig (same clone as 66674-1-PBS)

    IF Staining of RAW 264.7 using 66674-1-Ig (same clone as 66674-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using PARK2/Parkin antibody (66674-1-Ig, Clone: 2H5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PARK2/Parkin Monoclonal antibody, PBS Only

PARK2/Parkin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66674-1-PBS

产品说明书

CloneNo. 2H5A7

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Parkin, PARK2, Parkin RBR E3 ubiquitin-protein ligase, EC:2.3.2.31, E3 ubiquitin-protein ligase parkin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PARK2 Ready-To-Use IHC Kit. PARK2 primary antibody included.

产品信息

66674-1-PBS targets PARK2/Parkin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 PARK2/Parkin fusion protein Ag5179 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 Parkinson disease (autosomal recessive, juvenile) 2, parkin
别名 Parkin, PARK2, Parkin RBR E3 ubiquitin-protein ligase, EC:2.3.2.31, E3 ubiquitin-protein ligase parkin
计算分子量 52 kDa
观测分子量 42-52 kDa
GenBank蛋白编号BC022014
基因名称 Parkin
Gene ID (NCBI) 5071
RRIDAB_2882028
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60260
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing.