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PARP10 Polyclonal antibody

PARP10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26072-1-AP

Print datasheet

Synonyms

Poly [ADP-ribose] polymerase 10, PARP-10, PARP 10, EC:2.4.2.-, ARTD10

验证数据展示

  • WB analysis of Jurkat using 26072-1-AP

    WB analysis of Jurkat using 26072-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26072-1-AP (PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 26072-1-AP

    IP experiment of Jurkat using 26072-1-AP

    IP result of anti-PARP10 (IP:26072-1-AP, 4ug; Detection:26072-1-AP 1:500) with Jurkat cells lysate 3200ug.

  • IF Staining of MCF-7 using 26072-1-AP

    IF Staining of MCF-7 using 26072-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PARP10 antibody (26072-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IHC staining of human intrahepatic cholangiocarcinoma using 26072-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26072-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26072-1-AP (PARP10 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PARP10 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 26072-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26072-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26072-1-AP (PARP10 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PARP10 antibody included

经过测试的应用

Positive WB detected inJurkat cells, HL-60 cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PARP10 Ready-To-Use IHC Kit. PARP10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26072-1-AP targets PARP10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen PARP10 fusion protein Ag23457 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name poly (ADP-ribose) polymerase family, member 10
Synonyms Poly [ADP-ribose] polymerase 10, PARP-10, PARP 10, EC:2.4.2.-, ARTD10
Observed Molecular Weight 130-140 kDa
GenBank Accession NumberBC136542
Gene Symbol PARP10
Gene ID (NCBI) 84875
RRIDAB_2880362
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53GL7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PARP10 antibody 26072-1-APDownload protocol
IHC protocol for PARP10 antibody 26072-1-APDownload protocol
IF protocol for PARP10 antibody 26072-1-APDownload protocol
IP protocol for PARP10 antibody 26072-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols