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验证数据展示

  • WB analysis using 16692-1-AP

    WB analysis using 16692-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16692-1-AP (PARP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16692-1-AP

    IHC staining of human kidney using 16692-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 16692-1-AP

    IHC staining of human kidney using 16692-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PARP11 Polyclonal antibody

PARP11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16692-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA and More (1)

别名

C12orf6, PARP 11, PARP11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells, mouse heart tissue, mouse spleen tissue
Positive IHC detected inhuman kidney tissue, human placenta tissue, human colon tissue, human testis tissue, human skin tissue, human liver tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16692-1-AP targets PARP11 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, pig
免疫原 PARP11 fusion protein Ag10053 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly (ADP-ribose) polymerase family, member 11
别名 C12orf6, PARP 11, PARP11
计算分子量 331 aa, 39 kDa
观测分子量 30 kDa
GenBank蛋白编号BC017569
基因名称 PARP11
Gene ID (NCBI) 57097
RRIDAB_2158504
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NR21
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARP11, also named as ARTD11, belongs to the  ARTD/PARP family. PARP11 is a mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins. PARP11 plays a role in nuclear envelope stability and nuclear remodeling during spermiogenesis (PMID: 25043379). PARP11 has 4 isoforms with the molecular mass of 28-40 kDa.

实验方案

Product Specific Protocols
WB protocol for PARP11 antibody 16692-1-APDownload protocol
IHC protocol for PARP11 antibody 16692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,pigWB,IF

Front Cell Infect Microbiol

Identification of porcine PARP11 as a restricted factor for pseudorabies virus

Authors - Chunyun Qi
  • KD Validated
View Article