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PARP14 Recombinant antibody

PARP14 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241078C11

Cat no : 84039-6-RR

Print datasheet

Synonyms

PARP 14, KIAA1268, BAL2, 241078C11

验证数据展示

  • WB analysis using 84039-6-RR

    WB analysis using 84039-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84039-6-RR (PARP14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 84039-6-RR

    IHC staining of mouse colon using 84039-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84039-6-RR (PARP14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, U2OS cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84039-6-RR targets PARP14 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PARP14 fusion protein Ag35007 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name poly (ADP-ribose) polymerase family, member 14
Synonyms PARP 14, KIAA1268, BAL2, 241078C11
Calculated Molecular Weight203kd
Observed Molecular Weight200 kDa
GenBank Accession NumberNM_017554
Gene Symbol PARP14
Gene ID (NCBI) 54625
RRIDAB_3671606
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ460N5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARP14 (poly (ADP-ribose) polymerase family, member 14) is a PARP family member that has roles in DNA repair, B cell regulation, and focal adhesion (PMID: 25753673). The largest of all the human PARPs is PARP14. PARP14 has been reported to regulate several different pathways involved in immunity, inflammation, and genome stability (PMID: 37703374).

实验方案

Product Specific Protocols
WB protocol for PARP14 antibody 84039-6-RRDownload protocol
IHC protocol for PARP14 antibody 84039-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols