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PARP2 Polyclonal antibody

PARP2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 20555-1-AP

Print datasheet

Synonyms

ADPRT2, ADPRT-2, ADPRTL2, ARTD2, hPARP-2, pADPRT-2, PARP2, PARP-2, Poly [ADP-ribose] polymerase 2

验证数据展示

  • WB analysis using 20555-1-AP

    WB analysis using 20555-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20555-1-AP (PARP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 20555-1-AP

    IHC staining of mouse brain using 20555-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse brain tissue, mouse testis tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20555-1-AP targets PARP2 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name poly (ADP-ribose) polymerase 2
Synonyms ADPRT2, ADPRT-2, ADPRTL2, ARTD2, hPARP-2, pADPRT-2, PARP2, PARP-2, Poly [ADP-ribose] polymerase 2
Calculated Molecular Weight 66 kDa
Observed Molecular Weight60-66 kDa
GenBank Accession NumberNM_005484
Gene Symbol PARP2
Gene ID (NCBI) 10038
RRIDAB_10694417
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGN5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PRP2 catalyzes the reaction: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor. The antibody is specific to PARP2 (C-terminal). PARP2 can be detected as about 62 kDa (PMID: 10364231).

实验方案

Product Specific Protocols
WB protocol for PARP2 antibody 20555-1-APDownload protocol
IHC protocol for PARP2 antibody 20555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols