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验证数据展示

  • WB analysis using 19775-1-AP

    WB analysis using 19775-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 19775-1-AP (PARP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 19775-1-AP

    IHC staining of human stomach cancer using 19775-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19775-1-AP (PARP4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 19775-1-AP

    IF Staining of HepG2 using 19775-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed 48孔 HepG2 cells using PARP4 antibody (19775-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PARP4 Polyclonal antibody

PARP4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19775-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

PARP 4, p193, EC:2.4.2.-, ARTD4, ADPRTL1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in48孔 HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19775-1-AP targets PARP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly (ADP-ribose) polymerase family, member 4
别名 PARP 4, p193, EC:2.4.2.-, ARTD4, ADPRTL1
计算分子量 193 kDa
观测分子量193 kDa
GenBank蛋白编号NM_006437
基因名称 PARP4
Gene ID (NCBI) 143
RRIDAB_3085585
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKK3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARP4, also named as ADPRTL1, KIAA0177, PARPL, VPARP and PH5P, catalyzes the reaction: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.

实验方案

Product Specific Protocols
WB protocol for PARP4 antibody 19775-1-APDownload protocol
IHC protocol for PARP4 antibody 19775-1-APDownload protocol
IF protocol for PARP4 antibody 19775-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols