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  • Featured Product
  • KD/KO Validated

Alpha Parvin/Actopaxin Polyclonal antibody

Alpha Parvin/Actopaxin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 55268-1-AP

Print datasheet

Synonyms

Alpha parvin, Actopaxin, PARVA, α-Parvin, CH-ILKBP

验证数据展示

  • WB analysis of U2OS using 55268-1-AP

    WB analysis of U2OS using 55268-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 55268-1-AP

    WB analysis of HeLa using 55268-1-AP

    WB result of α-Parvin/Actopaxin antibody (55268-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-α-Parvin/Actopaxin transfected HeLa cells.

  • IF Staining of MDCK using 55268-1-AP

    IF Staining of MDCK using 55268-1-AP

    Immunofluorescent analysis of MDCK cells using 55268-1-AP (PARVA antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of U2OS using 55268-1-AP

    IP experiment of U2OS using 55268-1-AP

    IP result of anti-Alpha Parvin/Actopaxin (IP:55268-1-AP, 4ug; Detection:55268-1-AP 1:1000) with U2OS cells lysate 0 ug.

  • IHC staining of human stomach cancer using 55268-1-AP

    IHC staining of human stomach cancer using 55268-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 55268-1-AP (Alpha Parvin/Actopaxin antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PARVA antibody included

经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, mouse kidney tissue
Positive IP detected inU2OS cells
Positive IHC detected inhuman stomach cancer tissue, human heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells
Planning an IHC experiment? We recommend our IHCeasy PARVA Ready-To-Use IHC Kit. PARVA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55268-1-AP targets Alpha Parvin/Actopaxin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, CoIP
Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name parvin, alpha
Synonyms Alpha parvin, Actopaxin, PARVA, α-Parvin, CH-ILKBP
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberNM_018222
Gene Symbol Alpha Parvin/PARVA
Gene ID (NCBI) 55742
RRIDAB_10951112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVD7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

α-Parvin (PARVA), also named as MXRA2, Actopaxin and CH-ILKBP, belongs to the parvin family. It probably plays a role in the regulation of cell adhesion and cytoskeleton organization. (PMID:11134073) This antibody is specific to α-Parvin.

实验方案

Product Specific Protocols
WB protocol for Alpha Parvin/Actopaxin antibody 55268-1-APDownload protocol
IHC protocol for Alpha Parvin/Actopaxin antibody 55268-1-APDownload protocol
IF protocol for Alpha Parvin/Actopaxin antibody 55268-1-APDownload protocol
IP protocol for Alpha Parvin/Actopaxin antibody 55268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,CoIP

Proteome Sci

Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.

Authors - Lancia N F Darville
View Article
humanWB

Nat Commun

Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis

Authors - Yesheng Fu
View Article