• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HEK-293 using 14396-1-AP

    WB analysis of HEK-293 using 14396-1-AP

    WB result of PASK antibody (14396-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-PASK transfected HEK-293 cells.

  • WB analysis using 14396-1-AP

    WB analysis using 14396-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 14396-1-AP

    IP experiment of K-562 using 14396-1-AP

    IP result of anti-PASK (IP:14396-1-AP, 4ug; Detection:14396-1-AP 1:300) with K-562 cells lysate 4000 ug.

  • IHC staining of mouse testis using 14396-1-AP

    IHC staining of mouse testis using 14396-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14396-1-AP (PASK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 14396-1-AP

    IHC staining of mouse testis using 14396-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14396-1-AP (PASK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 14396-1-AP

    IF Staining of HeLa using 14396-1-AP

    Immunofluorescent analysis of HeLa cells using 14396-1-AP (PASK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

PASK Polyclonal antibody

PASK Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14396-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

PASKIN, PAS kinase, PAS domain-containing serine/threonine-protein kinase, KIAA0135, hPASK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, Jurkat cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse testis tissue, human prostate hyperplasia tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14396-1-AP targets PASK in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PASK fusion protein Ag5893 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PAS domain containing serine/threonine kinase
别名 PASKIN, PAS kinase, PAS domain-containing serine/threonine-protein kinase, KIAA0135, hPASK
计算分子量 143 kDa
观测分子量 180 kDa
GenBank蛋白编号BC063585
基因名称 PASK
Gene ID (NCBI) 23178
RRIDAB_2878053
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RG2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAS (Per-Arnt-Sim) domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. PAS domain-containing serine/threonine-protein kinase (PASK, also termed PAS-kinase or PASKIN) is an evolutionarily conserved nutrient responsive protein kinase. PASK links energy flux and protein synthesis in yeast and regulates glycogen synthesis in mammals. It has also been implicated in glucose-stimulated INS production in pancreatic beta-cells.

实验方案

Product Specific Protocols
WB protocol for PASK antibody 14396-1-APDownload protocol
IHC protocol for PASK antibody 14396-1-APDownload protocol
IF protocol for PASK antibody 14396-1-APDownload protocol
IP protocol for PASK antibody 14396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols