验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
60217-1-Ig targets PAX1-Specific in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | paired box 1 |
| 别名 | HUP48, paired box 1, Paired box protein Pax 1, PAX1 |
| 计算分子量 | 55 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | NM_006192 |
| 基因名称 | PAX1 |
| Gene ID (NCBI) | 5075 |
| RRID | AB_11183763 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P15863 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAX1, also named as HUP48, is a transcriptional activator. It may play a role in the formation of segmented structures of the embryo and may play an important role in the normal development of the vertebral column. It is a marker of the sclerotomal compartment. PAX1 was reported that, quantitative measurement of PAX1 hyper-methylation in cervical scrapings is highly sensitive and is more specific than HC2 in detection of cervical cancer. It is expressed by vSMC and pericytes during the earliest phases of vascular wall formation. This antibody is specific to PAX1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAX1-Specific antibody 60217-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |