PAX3 Polyclonal antibody
PAX3 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse skin tissue, HEK-293 cells |
Positive IP detected in | mouse skin tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
The immunogen of 21386-1-AP is PAX3 Fusion Protein expressed in E. coli.
Tested Applications | WB, IP, ELISA Application Description |
Cited Applications | IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | hamster |
Immunogen | PAX3 fusion protein Ag16021 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | paired box 3 |
Synonyms | CDHS, HUP2, paired box 3, Paired box protein Pax 3, PAX3, WS1 |
Calculated Molecular Weight | 484 aa, 53 kDa |
Observed Molecular Weight | 56-60 kDa |
GenBank Accession Number | BC101301 |
Gene Symbol | PAX3 |
Gene ID (NCBI) | 5077 |
RRID | AB_10732817 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P23760 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAX3, a transcription factor and multifunctional regulatory protein, is normally expressed during embryonic development. In the nervous system, PAX3 is involved in neural tube closure, neural crest development, and peripheral neuron differentiation. In the present study, PAX3 was reported as a novel regulator of GFAP transcription, and the overexpression and suppression of PAX3 could inhibit and promote NSC differentiation, respectively. In muscle development, PAX3 ensures the survival of myogenic progenitor cells with Pax3-expressing progenitors giving rise to both skeletal and smooth muscle cells. PAX3 also has a well-established role in the development of melanocytes during embryogenesis, and has recently been characterized as a molecular switch in the mature melanocyte. Mutations in PAX3 can cause Waardenburg syndrome.
实验方案
Product Specific Protocols | |
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WB protocol for PAX3 antibody 21386-1-AP | Download protocol |
IP protocol for PAX3 antibody 21386-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Birth Defects Res Cigarette smoke induced neural tube defects by down-regulating noggin expression. |