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PAX8 Recombinant antibody

PAX8 Uni-rAbTM Recombinant Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5D20

Cat no : 80022-1-RR

Print datasheet

Synonyms

paired box 8, Paired box protein Pax 8, PAX8

验证数据展示

  • IHC staining of human ovary tumor using 80022-1-RR

    IHC staining of human ovary tumor using 80022-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80022-1-RR (PAX8 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAX8 antibody included
  • IHC staining of human ovary tumor using 80022-1-RR

    IHC staining of human ovary tumor using 80022-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80022-1-RR (PAX8 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAX8 antibody included

经过测试的应用

Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PAX8 Ready-To-Use IHC Kit. PAX8 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80022-1-RR targets PAX8 in IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity Human
Immunogen PAX8 fusion protein Ag16019 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name paired box 8
Synonyms paired box 8, Paired box protein Pax 8, PAX8
Calculated Molecular Weight 450 aa, 48 kDa
GenBank Accession NumberBC001060
Gene Symbol PAX8
Gene ID (NCBI) 7849
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06710
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

实验方案

Product Specific Protocols
IHC protocol for PAX8 antibody 80022-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols