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验证数据展示

  • WB analysis of mouse kidney using 27891-1-AP

    WB analysis of mouse kidney using 27891-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27891-1-AP (PBLD antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 27891-1-AP

    WB analysis of mouse liver using 27891-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 27891-1-AP (PBLD antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 27891-1-AP

    IHC staining of human kidney using 27891-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27891-1-AP (PBLD antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 27891-1-AP

    IHC staining of human kidney using 27891-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27891-1-AP (PBLD antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PBLD Polyclonal antibody

PBLD Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27891-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse and More (1)

应用

WB, IHC, ELISA

别名

MAWBP, MAWD binding protein, MAWDBP, PBLD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, mouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27891-1-AP targets PBLD in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, Mouse
文献引用反应性human
免疫原 PBLD fusion protein Ag23591 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phenazine biosynthesis-like protein domain containing
别名 MAWBP, MAWD binding protein, MAWDBP, PBLD
观测分子量 30-32 kDa
GenBank蛋白编号BC009738
基因名称 PBLD
Gene ID (NCBI) 64081
RRIDAB_2881007
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP30039
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PBLD antibody 27891-1-APDownload protocol
IHC protocol for PBLD antibody 27891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

PBLD promotes IRF3 mediated the type I interferon (IFN-I) response and apoptosis to inhibit viral replication

Authors - Hongchao Zhu
View Article