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PBRM1 Recombinant antibody

PBRM1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240655H5

Cat no : 83677-2-RR

Print datasheet

Synonyms

PBRM 1, PB1, hPB1, BRG1-associated factor 180, BAF180

验证数据展示

  • WB analysis using 83677-2-RR

    WB analysis using 83677-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83677-2-RR (PBRM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83677-2-RR

    Affinity and Kinetic Characterization of 83677-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83677-2-RR against Human PBRM1 were performed. The affinity constant is 2.34 nM.

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, HeLa cellls, U2OS cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy PBRM1 Ready-To-Use IHC Kit. PBRM1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83677-2-RR is PBRM1 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PBRM1 fusion protein Ag33623 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name polybromo 1
Synonyms PBRM 1, PB1, hPB1, BRG1-associated factor 180, BAF180
Calculated Molecular Weight 180 kDa
Observed Molecular Weight180-195 kDa
GenBank Accession NumberBC015323
Gene Symbol PBRM1
Gene ID (NCBI) 55193
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86U86
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PBRM1, also named hPB1 BAF180 and Polybromo-1D, acts as a negative regulator of cell proliferation. PBRM1 is the second major mutated clear cell RCC cancer gene after VHL. PBRM1 loss is associated with low grade features.

实验方案

Product Specific Protocols
WB protocol for PBRM1 antibody 83677-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols