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PBX4 Polyclonal antibody

PBX4 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 22323-1-AP

Print datasheet

Synonyms

Homeobox protein PBX4, PBX4, pre B cell leukemia homeobox 4

验证数据展示

  • IP experiment of A2780 using 22323-1-AP

    IP experiment of A2780 using 22323-1-AP

    IP result of anti-PBX4 (IP:22323-1-AP, 4ug; Detection:22323-1-AP 1:300) with A2780 cells lysate 3600 ug.

  • WB analysis of HEK-293 using 22323-1-AP

    WB analysis of HEK-293 using 22323-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, SKOV-3 cells, A2780 cells, COLO 320 cells, Jurkat cells
Positive IP detected inA2780 cells
Planning an IHC experiment? We recommend our IHCeasy PBX4 Ready-To-Use IHC Kit. PBX4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22323-1-AP is PBX4 Fusion Protein expressed in E. coli.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen PBX4 fusion protein Ag17768 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pre-B-cell leukemia homeobox 4
Synonyms Homeobox protein PBX4, PBX4, pre B cell leukemia homeobox 4
Calculated Molecular Weight 374 aa, 41 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC141859
Gene Symbol PBX4
Gene ID (NCBI) 80714
RRIDAB_2879071
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9BYU1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pre-B-cell leukemia homeobox 4 (PBX4), also named as Homeobox protein PBX4, is one of the pbx gene encode homeodomain containing transcriptional regulators that interact with other proteins to control embryogenesis and tumorigenesis. Pbx 4 expression is confined to the testis, especially to spermatocytes in the pachytene stage of the first meiotic prophase. PBX4 may form heterodimers with other homeobox genes (hoxb1b or meis3) to specify different developmental fates during vertebrate embryogenesis (PMID:10679934). The trimeric complex containing Pbx4, Meis3, and Hoxb1b was also formed. The calculated molecular weight of PBX4 is about 41 kDa, but the molecular weight of heterodimers (PBX4 and hoxb1b) is about 70 kDa.

实验方案

Product Specific Protocols
WB protocol for PBX4 antibody 22323-1-APDownload protocol
IP protocol for PBX4 antibody 22323-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols