验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, HepG2 cells, rat liver tissue |
| Positive IP detected in | HepG2 cells, mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16588-1-AP targets Pyruvate Carboxylase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, bovine |
| 免疫原 | Pyruvate Carboxylase fusion protein Ag8857 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pyruvate carboxylase |
| 别名 | PC, PCB, pyruvate carboxykinase, pyruvate Carboxylase, Pyruvic carboxylase |
| 计算分子量 | 1178 aa, 130 kDa |
| 观测分子量 | 125-130 kDa |
| GenBank蛋白编号 | BC011617 |
| 基因名称 | Pyruvate Carboxylase |
| Gene ID (NCBI) | 5091 |
| RRID | AB_1851513 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P11498 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PC(pyruvate carboxylase) is a member of the family of biotin-dependent carboxylases and is found widely among eukaryotic tissues and in many prokaryotic species. It catalyses the ATP-dependent carboxylation of pyruvate to form oxaloacetate which may be utilised in the synthesis of glucose, fat, some amino acids or their derivatives and several neurotransmitters. Diabetes and hyperthyroidism increase the level of expression of pyruvate carboxylase in the long term, while its activity in the short term is controlled by the intramitochondrial concentrations of acetyl-CoA and pyruvate(PMID:9597748).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Pyruvate Carboxylase antibody 16588-1-AP | Download protocol |
| IHC protocol for Pyruvate Carboxylase antibody 16588-1-AP | Download protocol |
| IF protocol for Pyruvate Carboxylase antibody 16588-1-AP | Download protocol |
| IP protocol for Pyruvate Carboxylase antibody 16588-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cells | ||
Nat Commun SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition. | ||
Nat Commun Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise. | ||
Nat Commun The long noncoding RNA ADIPINT regulates human adipocyte metabolism via pyruvate carboxylase. | ||
Cancer Res A short isoform of spermatogenic enzyme GAPDHS functions as a metabolic switch and limits metastasis in melanoma. | ||
Elife Differential requirements for mitochondrial electron transport chain components in the adult murine liver |
