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Pyruvate Carboxylase Monoclonal antibody
Pyruvate Carboxylase Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Rat , mouse and More (1)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
4F5D1
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, HSC-T6 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PC Ready-To-Use IHC Kit. PC primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| RIP | See 1 publications below |
产品信息
66615-1-Ig targets Pyruvate Carboxylase in WB, IHC, RIP, ELISA applications and shows reactivity with Human, Rat , mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, RIP |
| Tested Reactivity | Human, Rat , mouse |
| Cited Reactivity | human, mouse |
| Immunogen | Pyruvate Carboxylase fusion protein Ag8846 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | pyruvate carboxylase |
| Synonyms | PC, PCB, pyruvate carboxykinase, pyruvate carboxylase, Pyruvic carboxylase |
| Calculated Molecular Weight | 1178 aa, 130 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC011617 |
| Gene Symbol | Pyruvate Carboxylase |
| Gene ID (NCBI) | 5091 |
| RRID | AB_2881975 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P11498 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Pyruvate Carboxylase antibody 66615-1-Ig | Download protocol |
| IHC protocol for Pyruvate Carboxylase antibody 66615-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption | ||
Front Oncol piR-hsa-211106 Inhibits the Progression of Lung Adenocarcinoma Through Pyruvate Carboxylase and Enhances Chemotherapy Sensitivity. | ||
Elife Mitochondrial redox adaptations enable alternative aspartate synthesis in SDH-deficient cells
|
