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PCBD1 Polyclonal antibody

PCBD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15702-1-AP

Print datasheet

Synonyms

PCD, PCBD, EC:4.2.1.96, Dimerization cofactor of hepatocyte nuclear factor 1-alpha, DCOH

验证数据展示

  • WB analysis using 15702-1-AP

    WB analysis using 15702-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15702-1-AP (PCBD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15702-1-AP

    IF Staining of HeLa using 15702-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PCBD1 antibody (15702-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • IHC staining of human liver cancer using 15702-1-AP

    IHC staining of human liver cancer using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCBD1 antibody included

经过测试的应用

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PCBD1 Ready-To-Use IHC Kit. PCBD1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15702-1-AP targets PCBD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PCBD1 fusion protein Ag8331 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha
Synonyms PCD, PCBD, EC:4.2.1.96, Dimerization cofactor of hepatocyte nuclear factor 1-alpha, DCOH
Calculated Molecular Weight 104 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC006324
Gene Symbol PCBD1
Gene ID (NCBI) 5092
RRIDAB_2267945
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61457
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCBD1, also known as DCOH and PCBD, belongs to the pterin-4-alpha-carbinolamine dehydratase family. PCBD1 is a bifunctional protein that acts as an enzyme in the regeneration of cofactor tetrahydrobiopterin (BH4), crucial for the function of aromatic amino acid hydroxylases, and as a dimerization cofactor of transcription factors HNF1A and HNF1B, important in liver and pancreas development and function. PCBD1 is expressed in the developing pancreas. The calculated molecular weight of PCBD1 is 12 kDa (PMID: 24204001, 24848070).

实验方案

Product Specific Protocols
WB protocol for PCBD1 antibody 15702-1-APDownload protocol
IHC protocol for PCBD1 antibody 15702-1-APDownload protocol
IF protocol for PCBD1 antibody 15702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols