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验证数据展示

  • WB analysis using 15702-1-AP

    WB analysis using 15702-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15702-1-AP (PCBD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15702-1-AP

    IF Staining of HeLa using 15702-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PCBD1 antibody (15702-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • IHC staining of human liver cancer using 15702-1-AP

    IHC staining of human liver cancer using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCBD1 antibody included

PCBD1 Polyclonal antibody

PCBD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15702-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

PCD, PCBD, EC:4.2.1.96, Dimerization cofactor of hepatocyte nuclear factor 1-alpha, DCOH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PCBD1 Ready-To-Use IHC Kit. PCBD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15702-1-AP targets PCBD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PCBD1 fusion protein Ag8331 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha
别名 PCD, PCBD, EC:4.2.1.96, Dimerization cofactor of hepatocyte nuclear factor 1-alpha, DCOH
计算分子量 104 aa, 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC006324
基因名称 PCBD1
Gene ID (NCBI) 5092
RRIDAB_2267945
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61457
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCBD1, also known as DCOH and PCBD, belongs to the pterin-4-alpha-carbinolamine dehydratase family. PCBD1 is a bifunctional protein that acts as an enzyme in the regeneration of cofactor tetrahydrobiopterin (BH4), crucial for the function of aromatic amino acid hydroxylases, and as a dimerization cofactor of transcription factors HNF1A and HNF1B, important in liver and pancreas development and function. PCBD1 is expressed in the developing pancreas. The calculated molecular weight of PCBD1 is 12 kDa (PMID: 24204001, 24848070).

实验方案

Product Specific Protocols
WB protocol for PCBD1 antibody 15702-1-APDownload protocol
IHC protocol for PCBD1 antibody 15702-1-APDownload protocol
IF protocol for PCBD1 antibody 15702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols