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PCBP3 Polyclonal antibody

PCBP3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16141-1-AP

Print datasheet

Synonyms

PCBP3-overlapping transcript 1, PCBP3-overlapping transcript, PCBP3-OT1, PCBP3OT, Alpha-CP3

验证数据展示

  • WB analysis using 16141-1-AP

    WB analysis using 16141-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16141-1-AP (PCBP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 16141-1-AP

    IF Staining of MCF-7 using 16141-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PCBP3 antibody (16141-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inHT-1080 cells, K-562 cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16141-1-AP targets PCBP3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen PCBP3 fusion protein Ag9202 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name poly(rC) binding protein 3
Synonyms PCBP3-overlapping transcript 1, PCBP3-overlapping transcript, PCBP3-OT1, PCBP3OT, Alpha-CP3
Calculated Molecular Weight 313 aa, 33 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC012061
Gene Symbol PCBP3
Gene ID (NCBI) 54039
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP57721
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCBP3 is a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions.

实验方案

Product Specific Protocols
WB protocol for PCBP3 antibody 16141-1-APDownload protocol
IF protocol for PCBP3 antibody 16141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols