验证数据展示
经过测试的应用
Positive WB detected in | HT-1080 cells, K-562 cells, MCF-7 cells |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16141-1-AP targets PCBP3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | PCBP3 fusion protein Ag9202 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | poly(rC) binding protein 3 |
Synonyms | PCBP3-overlapping transcript 1, PCBP3-overlapping transcript, PCBP3-OT1, PCBP3OT, Alpha-CP3 |
Calculated Molecular Weight | 313 aa, 33 kDa |
Observed Molecular Weight | 37 kDa |
GenBank Accession Number | BC012061 |
Gene Symbol | PCBP3 |
Gene ID (NCBI) | 54039 |
RRID | AB_3085481 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P57721 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PCBP3 is a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. PCBP3 is broadly expressed in various tissues, including the testis and endometrium. It has been associated with diseases such as dementia.
实验方案
Product Specific Protocols | |
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WB protocol for PCBP3 antibody 16141-1-AP | Download protocol |
IF protocol for PCBP3 antibody 16141-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |