PCCA Polyclonal antibody
PCCA Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse heart tissue, L02 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue, mouse skeletal muscle tissue, rat heart tissue |
Positive IP detected in | L02 cells |
Positive IHC detected in | human liver cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21988-1-AP targets PCCA in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | PCCA fusion protein Ag17271 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | propionyl Coenzyme A carboxylase, alpha polypeptide |
Synonyms | Propionyl-CoA carboxylase alpha chain, mitochondrial, Propanoyl-CoA:carbon dioxide ligase subunit alpha, PCCase subunit alpha, EC:6.4.1.3 |
Calculated Molecular Weight | 80 kDa |
Observed Molecular Weight | 70 kDa |
GenBank Accession Number | BC000140 |
Gene Symbol | PCCA |
Gene ID (NCBI) | 5095 |
RRID | AB_2878963 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P05165 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PCCA antibody 21988-1-AP | Download protocol |
IHC protocol for PCCA antibody 21988-1-AP | Download protocol |
IP protocol for PCCA antibody 21988-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Dual mRNA therapy restores metabolic function in long-term studies in mice with propionic acidemia. | ||
J Cell Biol CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs. | ||
Mol Genet Metab Metabolic perturbations mediated by propionyl-CoA accumulation in organs of mouse model of propionic acidemia. | ||
Hum Gene Ther Effects of Adeno-associated Virus Serotype and Tissue-specific Expression on Circulating Biomarkers of Propionic Acidemia. | ||
Hum Gene Ther Long-term Sex-Biased Correction of Circulating Propionic Acidemia Disease Markers by Adeno-Associated Virus Vectors. | ||
Biology (Basel) In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome. |