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PCDH11X Polyclonal antibody

PCDH11X Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20070-1-AP

Print datasheet

Synonyms

PCDHY, PCDH-X, PCDHX, PCDH11, PCDH X

验证数据展示

  • WB analysis of human brain using 20070-1-AP

    WB analysis of human brain using 20070-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20070-1-AP (PCDH11X antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 20070-1-AP

    IF Staining of HepG2 using 20070-1-AP

    Immunofluorescent analysis of HepG2 cells, using PCDH11X antibody 20070-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human gliomas using 20070-1-AP

    IHC staining of human gliomas using 20070-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20070-1-AP (PCDH11X antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 20070-1-AP

    IHC staining of human gliomas using 20070-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20070-1-AP (PCDH11X antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman gliomas tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20070-1-AP targets PCDH11X in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protocadherin 11 X-linked
Synonyms PCDHY, PCDH-X, PCDHX, PCDH11, PCDH X
Calculated Molecular Weight 148 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberNM_032968
Gene Symbol PCDH11X
Gene ID (NCBI) 27328
RRIDAB_10667457
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZA7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCDH11X, also named as KIAA1326, PCDH11 and PCDHX, is a potential calcium-dependent cell-adhesion protein. The antibody recognizes isoforms1 and isoform6 of PCDH11X. The antibody has no cross reaction to PCDH11Y.

实验方案

Product Specific Protocols
WB protocol for PCDH11X antibody 20070-1-APDownload protocol
IHC protocol for PCDH11X antibody 20070-1-APDownload protocol
IF protocol for PCDH11X antibody 20070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols