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验证数据展示

  • WB analysis using 20719-1-AP

    WB analysis using 20719-1-AP

    mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 20719-1-AP (PCDH15 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 20719-1-AP

    IF Staining of HEK-293 using 20719-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PCDH15 antibody (20719-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

PCDH15 Polyclonal antibody

PCDH15 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20719-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

DFNB23, PCDH15, protocadherin 15, USH1F

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse liver tissue, rat brain tissue
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20719-1-AP targets PCDH15 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protocadherin 15
别名 DFNB23, PCDH15, protocadherin 15, USH1F
计算分子量 216 kDa
观测分子量120 kDa, 50 kDa
GenBank蛋白编号NM_033056
基因名称 PCDH15
Gene ID (NCBI) 65217
RRIDAB_3085617
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96QU1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protocadherin-15 (PCDH15) belongs to the cadherin superfamily that mediates calcium-dependent cell-cell adhesion and plays an important role in the maintenance of normal tissue architecture. A homozygous variant in PCDH15 results in Usher syndrome type 1F (USH1F) which leads to congenital hearing loss and syndromicretinitis pigmentosa (PMID: 38014037). Multiple isoforms of PCDH15 have been described, including 216-250 kDa (full-length), 150-180 kDa, 100-120 kDa, 50-65 kDa, and 25-40 kDa (PMID: 22363448; 15928608; 14570705; 23035094; 28883094).

实验方案

Product Specific Protocols
WB protocol for PCDH15 antibody 20719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols