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PCDH24 Polyclonal antibody

PCDH24 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27103-1-AP

Print datasheet

Synonyms

Protocadherin-24, PC-LKC, PCLKC, PC LKC, CDHR2

验证数据展示

  • WB analysis of mouse liver using 27103-1-AP

    WB analysis of mouse liver using 27103-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 27103-1-AP (PCDH24 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 27103-1-AP

    IHC staining of human kidney using 27103-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCDH24 antibody included
  • IHC staining of human kidney using 27103-1-AP

    IHC staining of human kidney using 27103-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCDH24 antibody included

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue, human liver tissue, human small intestine tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PCDH24 Ready-To-Use IHC Kit. PCDH24 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27103-1-AP targets PCDH24 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen PCDH24 fusion protein Ag25840 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protocadherin 24
Synonyms Protocadherin-24, PC-LKC, PCLKC, PC LKC, CDHR2
Calculated Molecular Weight 1310 aa, 142 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC130282
Gene Symbol PCDH24
Gene ID (NCBI) 54825
RRIDAB_2880757
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYE9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protocadherin-24 (PCDH24, also known as CDHR2 and protocadherin LKC) was originally identified as a novel member of the human non-classical cadherin family. PCDH24 may play a role in cell-cell adhesion and contact inhibition in epithelial cells. PCDH24 expression is markedly reduced in cancers derived from liver and colon tissues, indicating that PCDH24 may play a role as a tumor suppressor protein (PMID: 23115611). The calculated molecular weight of PCDH24 is 142 kDa.

实验方案

Product Specific Protocols
WB protocol for PCDH24 antibody 27103-1-APDownload protocol
IHC protocol for PCDH24 antibody 27103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Transl Androl Urol

Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples.

Authors - Xuechao Li
View Article