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PCDH8 Polyclonal antibody

PCDH8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14000-1-AP

Print datasheet

Synonyms

Protocadherin-8, Arcadlin

验证数据展示

  • WB analysis using 14000-1-AP

    WB analysis using 14000-1-AP

    mouse brain and unboiled mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 14000-1-AP (PCDH8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14000-1-AP

    WB analysis using 14000-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14000-1-AP (PCDH8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, L02 cells, Neuro-2a cells, U-251 cells, U-87 MG cells, unboiled mouse brain

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14000-1-AP targets PCDH8 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PCDH8 fusion protein Ag5090 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protocadherin 8
Synonyms Protocadherin-8, Arcadlin
Calculated Molecular Weight 1070 aa, 113 kDa
Observed Molecular Weight104 kDa,113 kDa
GenBank Accession NumberBC036025
Gene Symbol PCDH8
Gene ID (NCBI) 5100
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95206
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protocadherin-8 (PCDH8) is a member of the protocadherin family that belongs to the cadherin superfamily. Protocadherins are calcium-dependent adhesion proteins and have been implicated in neural cell-cell interactions. They are abundantly expressed in the central nervous system during embryonic development and in adulthood (PMID: 24214103). PCDH8 is a tumor suppressor involved in tumorigenesis.

实验方案

Product Specific Protocols
WB protocol for PCDH8 antibody 14000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols