验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, Raji cells, human heart tissue, HeLa cells, human brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10127-2-AP targets PCDHA2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | PCDHA2 fusion protein Ag0173 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protocadherin alpha 2 |
| 别名 | PCDH alpha 2, PCDH ALPHA2, PCDHA2, protocadherin alpha 2 |
| 计算分子量 | 102 kDa |
| 观测分子量 | 102 kDa, 80 kDa |
| GenBank蛋白编号 | BC003126 |
| 基因名称 | PCDHA2 |
| Gene ID (NCBI) | 56146 |
| RRID | AB_2158964 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y5H9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β- and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). PCDHA2 (protocadherin alpha 2) is a single-pass type I membrane protein containing 6 cadherin domains. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. Three isoforms produced by alternative splicing have been described (UniProt).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCDHA2 antibody 10127-2-AP | Download protocol |
| IP protocol for PCDHA2 antibody 10127-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
