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PCDHA3 Polyclonal antibody

PCDHA3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18803-1-AP

Print datasheet

Synonyms

PCDH alpha 3, PCDH ALPHA3, PCDHA3, protocadherin alpha 3

验证数据展示

  • WB analysis using 18803-1-AP

    WB analysis using 18803-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 18803-1-AP (PCDHA3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 18803-1-AP

    IHC staining of mouse brain using 18803-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18803-1-AP (PCDHA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCDHA3 antibody included
  • IHC staining of mouse brain using 18803-1-AP

    IHC staining of mouse brain using 18803-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18803-1-AP (PCDHA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCDHA3 antibody included

经过测试的应用

Positive WB detected inA549 cells, mouse colon tissue
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PCDHA3 Ready-To-Use IHC Kit. PCDHA3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18803-1-AP targets PCDHA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protocadherin alpha 3
Synonyms PCDH alpha 3, PCDH ALPHA3, PCDHA3, protocadherin alpha 3
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC129807
Gene Symbol PCDHA3
Gene ID (NCBI) 56145
RRIDAB_10596786
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5H8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCDHA3 is a potential calcium-dependent cell-adhesion protein. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. The antibody can recognize both the isoforms of PCDHA3.

实验方案

Product Specific Protocols
WB protocol for PCDHA3 antibody 18803-1-APDownload protocol
IHC protocol for PCDHA3 antibody 18803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols