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PCDHA5 Polyclonal antibody

PCDHA5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15270-1-AP

Print datasheet

Synonyms

PCDH ALPHA5, PCDH alpha 5, CRNR6, CNRS6, CNRN6

验证数据展示

  • WB analysis using 15270-1-AP

    WB analysis using 15270-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15270-1-AP (PCDHA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 15270-1-AP

    IHC staining of mouse cerebellum using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCDHA5 antibody included
  • IHC staining of mouse cerebellum using 15270-1-AP

    IHC staining of mouse cerebellum using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCDHA5 antibody included
  • IHC staining of mouse cerebellum using 15270-1-AP

    IHC staining of mouse cerebellum using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCDHA5 antibody included

经过测试的应用

Positive WB detected inunboiled mouse brain tissue, human brain tissue, unboiled rat brain tissue
Positive IHC detected inmouse cerebellum tissue, human brain tissue, human gliomas tissue, human lung tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PCDHA5 Ready-To-Use IHC Kit. PCDHA5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15270-1-AP targets PCDHA5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PCDHA5 fusion protein Ag7376 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protocadherin alpha 5
Synonyms PCDH ALPHA5, PCDH alpha 5, CRNR6, CNRS6, CNRN6
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC033735
Gene Symbol PCDHA5
Gene ID (NCBI) 56143
RRIDAB_2283415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5H7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PCDHA5 antibody 15270-1-APDownload protocol
IHC protocol for PCDHA5 antibody 15270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols