验证数据展示
经过测试的应用
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18075-1-AP targets PCDHA9 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, IF-P, IP, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | PCDHA9 fusion protein Ag12767 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | protocadherin alpha 9 |
| Synonyms | Protocadherin alpha-9, PCDH-alpha-9, KIAA0345 |
| Calculated Molecular Weight | 950 aa, 102 kDa |
| Observed Molecular Weight | 91 kDa |
| GenBank Accession Number | BC104802 |
| Gene Symbol | PCDHA9 |
| Gene ID (NCBI) | 9752 |
| RRID | AB_2878491 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5H5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β- and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). PCDHA9 belongs to the α-protocadherin (PCDHA) cluster. A homozygous variant in PCDHA9 has been found in three unrelated Chinese ALS patients, suggesting PCDHA9 as a candidate gene for amyotrophic lateral sclerosis(PMID: 38467605).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PCDHA9 antibody 18075-1-AP | Download protocol |
| IF protocol for PCDHA9 antibody 18075-1-AP | Download protocol |
| IP protocol for PCDHA9 antibody 18075-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
