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Conjugate
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PCDHGB3 Polyclonal antibody

PCDHGB3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21204-1-AP

Print datasheet

Synonyms

Protocadherin gamma-B3, PCDH-gamma-B3

验证数据展示

  • WB analysis of human brain using 21204-1-AP

    WB analysis of human brain using 21204-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21204-1-AP (PCDHGB3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 21204-1-AP

    IF Staining of HeLa using 21204-1-AP

    Immunofluorescent analysis of Hela cells, using PCDHGB3 antibody 21204-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inhuman brain tissue, Neuro-2a cells
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21204-1-AP targets PCDHGB3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PCDHGB3 fusion protein Ag15569 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protocadherin gamma subfamily B, 3
Synonyms Protocadherin gamma-B3, PCDH-gamma-B3
Calculated Molecular Weight 929 aa, 101 kDa
Observed Molecular Weight 89 kDa
GenBank Accession NumberBC150172
Gene Symbol PCDHGB3
Gene ID (NCBI) 56102
RRIDAB_10858620
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5G1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PCDHGB3 antibody 21204-1-APDownload protocol
IF protocol for PCDHGB3 antibody 21204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols