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验证数据展示

  • IHC staining of mouse kidney using 21204-1-AP

    IHC staining of mouse kidney using 21204-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21204-1-AP (PCDHGB3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 21204-1-AP

    IHC staining of mouse kidney using 21204-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21204-1-AP (PCDHGB3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 21204-1-AP

    IF Staining of HeLa using 21204-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PCDHGB3 antibody (21204-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

PCDHGB3 Polyclonal antibody

PCDHGB3 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 21204-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

Protocadherin gamma-B3, PCDH-gamma-B3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21204-1-AP targets PCDHGB3 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PCDHGB3 fusion protein Ag15569 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protocadherin gamma subfamily B, 3
别名 Protocadherin gamma-B3, PCDH-gamma-B3
计算分子量 929 aa, 101 kDa
观测分子量 89 kDa
GenBank蛋白编号BC150172
基因名称 PCDHGB3
Gene ID (NCBI) 56102
RRIDAB_10858620
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5G1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for PCDHGB3 antibody 21204-1-APDownload protocol
IF protocol for PCDHGB3 antibody 21204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols