• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PCF11 Recombinant antibody

PCF11 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241213F9

Cat no : 84081-4-RR

Print datasheet

Synonyms

Pre-mRNA cleavage complex II protein Pcf11, Pre-mRNA cleavage complex 2 protein Pcf11, KIAA0824, 241213F9

验证数据展示

  • WB analysis using 84081-4-RR

    WB analysis using 84081-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84081-4-RR (PCF11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of U-2 OS using 84081-4-RR

    FC experiment of U-2 OS using 84081-4-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug PCF11 Recombinant antibody (84081-4-RR, Clone:241213F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, Jurkat cells, Neuro-2a cells, RAW 264.7 cells,
Positive FC (Intra) detected inU-2 OS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84081-4-RR targets PCF11 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen PCF11 fusion protein Ag20263 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)
Synonyms Pre-mRNA cleavage complex II protein Pcf11, Pre-mRNA cleavage complex 2 protein Pcf11, KIAA0824, 241213F9
Calculated Molecular Weight 1555 aa, 173 kDa
Observed Molecular Weight180~200 kDa
GenBank Accession NumberBC146778
Gene Symbol PCF11
Gene ID (NCBI) 51585
RRIDAB_3671649
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO94913
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

In Saccharomyces cerevisiae, the cleavage/polyadenylation factor Pcf11 is a crucial regulatory factor required for recruiting polyadenylation machinery to elongating RNA polymerase II (RNAPII), and is necessary for correct transcriptional termination. Pcf11 (PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)), is a 1,555 amino acid nuclear protein that is a component of pre-mRNA cleavage complex II. It is suggested that Pcf11 is capable of promoting the dissociation of Pol II elongation complexes from DNA. Pcf11 contains a CTD-interaction domain that binds in a phospho-dependent manner to the heptad repeats within the RNA polymerase II CTD. The gene encoding Pcf11 is located on human chromosoem 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11.

实验方案

Product Specific Protocols
WB protocol for PCF11 antibody 84081-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols