验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15581-1-AP targets PCGF1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | PCGF1 fusion protein Ag7983 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | polycomb group ring finger 1 |
| 别名 | RNF3A 2, RING finger protein 68, polycomb group ring finger 1, NSPc1, Nervous system Polycomb-1 |
| 计算分子量 | 30 kDa |
| 观测分子量 | 30-31 kDa |
| GenBank蛋白编号 | BC004952 |
| 基因名称 | PCGF1 |
| Gene ID (NCBI) | 84759 |
| RRID | AB_3669212 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BSM1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PCGF1 (Polycomb group RING finger protein 1), also known as NSPC1. It is expected to be located in the nucleus. Human and mouse NSPc1 genes encode proteins with an N-terminal RING finger domain and share homology with Drosophila melanogaster lethal(3)73Ah and the mammalian Mel18 and Bmi1 genes. Transcripts are observed at 10 dpc in the otic vesicle, urogenital bud and dorsal root ganglia. At 11.5 dpc, transcripts are present in a subset of neural crest cell derivatives of the peripheral nervous system, and in the neural tube. NSPc1 expression is ubiquitous in adult tissue (PMID: 11287196). The molecular weight of PCGF1is 30 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCGF1 antibody 15581-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |