验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HEK-293T cells, PC-13 cells, mouse liver tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4800 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24103-1-AP targets PCGF6 in WB, IHC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA, FC (Intra) Application Description |
| 文献引用应用 | WB, IP, CoIP, ChIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | PCGF6 fusion protein Ag21124 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | polycomb group ring finger 6 |
| 别名 | RNF134, PCGF 6, Mel18 and Bmi1-like RING finger, MBLR |
| 计算分子量 | 352 aa, 39 kDa |
| 观测分子量 | 40-50 kDa |
| GenBank蛋白编号 | BC010235 |
| 基因名称 | PCGF6 |
| Gene ID (NCBI) | 84108 |
| RRID | AB_2737369 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q9BYE7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PCGF6, also named as MBLR or RNF134, is a 350 amino acid protein, which contains one RING-type zinc finger. PCGF6 localizes in the nucleus and is widely expressed in many tissues. PCGF6 as a transcriptional repressor may modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCGF6 antibody 24103-1-AP | Download protocol |
| IHC protocol for PCGF6 antibody 24103-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell SUMO Safeguards Somatic and Pluripotent Cell Identities by Enforcing Distinct Chromatin States. | ||
Sci Adv The SAM domain-containing protein 1 (SAMD1) acts as a repressive chromatin regulator at unmethylated CpG islands. | ||
Nat Commun E2F6 initiates stable epigenetic silencing of germline genes during embryonic development. | ||
Nat Commun Repression of germline genes by PRC1.6 and SETDB1 in the early embryo precedes DNA methylation-mediated silencing.
| ||
Nat Commun PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.
| ||
Elife Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness. |