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PCID2 Polyclonal antibody

PCID2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 26638-1-AP

Print datasheet

Synonyms

CSN12 like protein, F10, PCI domain containing 2, PCID2

验证数据展示

  • IHC staining of human breast hyperplasia using 26638-1-AP

    IHC staining of human breast hyperplasia using 26638-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 26638-1-AP (PCID2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCID2 antibody included
  • IHC staining of human breast cancer using 26638-1-AP

    IHC staining of human breast cancer using 26638-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26638-1-AP (PCID2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCID2 antibody included
  • IHC staining of human breast hyperplasia using 26638-1-AP

    IHC staining of human breast hyperplasia using 26638-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 26638-1-AP (PCID2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCID2 antibody included

经过测试的应用

Positive IHC detected inhuman breast hyperplasia tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

This antibody is recommended for IHC.

Planning an IHC experiment? We recommend our IHCeasy PCID2 Ready-To-Use IHC Kit. PCID2 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26638-1-AP is PCID2 Fusion Protein expressed in E. coli.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human
Immunogen PCID2 fusion protein Ag24417 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PCI domain containing 2
Synonyms CSN12 like protein, F10, PCI domain containing 2, PCID2
Calculated Molecular Weight 399 aa, 46 kDa
GenBank Accession NumberBC031246
Gene Symbol PCID2
Gene ID (NCBI) 55795
RRIDAB_2918105
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5JVF3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human PCID2, is characterized by its PCI interaction domain and its involvement in the mammalian transcription export complex (TREX-2). PCID2 colocalizes with gamma-tubulin and centrin-2 at the centrosome of HeLa cells. It's required for B-cell survival through the regulation of the expression of cell-cycle checkpoint MAD2L1 protein during B cell differentiation. PCID2 has some isoforms with MW 43-46 kDa and 52 kDa.

实验方案

Product Specific Protocols
IHC protocol for PCID2 antibody 26638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols