验证数据展示
经过测试的应用
Positive IHC detected in | human breast hyperplasia tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
This antibody is recommended for IHC.
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26638-1-AP targets PCID2 in IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | PCID2 fusion protein Ag24417 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | PCI domain containing 2 |
别名 | CSN12 like protein, F10, PCI domain containing 2, PCID2 |
计算分子量 | 399 aa, 46 kDa |
GenBank蛋白编号 | BC031246 |
基因名称 | PCID2 |
Gene ID (NCBI) | 55795 |
RRID | AB_2918105 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q5JVF3 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human PCID2, is characterized by its PCI interaction domain and its involvement in the mammalian transcription export complex (TREX-2). PCID2 colocalizes with gamma-tubulin and centrin-2 at the centrosome of HeLa cells. It's required for B-cell survival through the regulation of the expression of cell-cycle checkpoint MAD2L1 protein during B cell differentiation. PCID2 has some isoforms with MW 43-46 kDa and 52 kDa.
实验方案
Product Specific Protocols | |
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IHC protocol for PCID2 antibody 26638-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |