验证数据展示
经过测试的应用
Positive WB detected in | Ramos cells, HepG2 cells, Jurkat cells, HEK-293T cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16082-1-AP targets PCIF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IF, IP |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse, pig |
免疫原 | PCIF1 fusion protein Ag9080 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | PDX1 C-terminal inhibiting factor 1 |
别名 | C20orf67, CAPAM, Cap-specific adenosine methyltransferase, EC:2.1.1.62, hCAPAM |
计算分子量 | 704 aa, 81 kDa |
观测分子量 | 81 kDa |
GenBank蛋白编号 | BC013365 |
基因名称 | PCIF1 |
Gene ID (NCBI) | 63935 |
RRID | AB_2159996 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9H4Z3 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for PCIF1 antibody 16082-1-AP | Download protocol |
IHC protocol for PCIF1 antibody 16082-1-AP | Download protocol |
IF protocol for PCIF1 antibody 16082-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits | ||
Nat Commun HIV reprograms host m 6 Am RNA methylome by viral Vpr protein-mediated degradation of PCIF1 | ||
Cell Rep Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex. | ||
EMBO J Role of PCIF1-mediated 5\'-cap N6-methyladeonsine mRNA methylation in colorectal cancer and anti-PD-1 immunotherapy | ||
Proc Natl Acad Sci U S A PCIF1-mediated deposition of 5'-cap N6,2'-O-dimethyladenosine in ACE2 and TMPRSS2 mRNA regulates susceptibility to SARS-CoV-2 infection |