PCIF1 Polyclonal antibody

PCIF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16082-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (2)

C20orf67, CAPAM, Cap-specific adenosine methyltransferase, EC:2.1.1.62, hCAPAM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inRamos cells, HepG2 cells, Jurkat cells, HEK-293T cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16082-1-AP targets PCIF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse
文献引用反应性human, mouse, pig
免疫原 PCIF1 fusion protein Ag9080 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PDX1 C-terminal inhibiting factor 1
别名 C20orf67, CAPAM, Cap-specific adenosine methyltransferase, EC:2.1.1.62, hCAPAM
计算分子量 704 aa, 81 kDa
观测分子量 81 kDa
GenBank蛋白编号BC013365
基因名称 PCIF1
Gene ID (NCBI) 63935
RRIDAB_2159996
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H4Z3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PCIF1 antibody 16082-1-APDownload protocol
IHC protocol for PCIF1 antibody 16082-1-APDownload protocol
IF protocol for PCIF1 antibody 16082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IP

Mol Cell

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits

Authors - Yuanjun Li
humanWB

Nat Commun

HIV reprograms host m 6 Am RNA methylome by viral Vpr protein-mediated degradation of PCIF1

Authors - Qiong Zhang
pigWB

Cell Rep

Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex.

Authors - Robert J J Jansens
human,mouseWB,IF

EMBO J

Role of PCIF1-mediated 5\'-cap N6-methyladeonsine mRNA methylation in colorectal cancer and anti-PD-1 immunotherapy

Authors - Lingling Wang
humanWB

Proc Natl Acad Sci U S A

PCIF1-mediated deposition of 5'-cap N6,2'-O-dimethyladenosine in ACE2 and TMPRSS2 mRNA regulates susceptibility to SARS-CoV-2 infection

Authors - Lingling Wang
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