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验证数据展示

  • WB analysis using 67676-1-Ig (same clone as 67676-1-PBS)

    WB analysis using 67676-1-Ig (same clone as 67676-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67676-1-Ig (same clone as 67676-1-PBS)

    IHC staining of human lung cancer using 67676-1-Ig (same clone as 67676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCK2 antibody included
  • IP experiment of HepG2 using 67676-1-Ig (same clone as 67676-1-PBS)

    IP experiment of HepG2 using 67676-1-Ig (same clone as 67676-1-PBS)

    IP result of anti-PCK2 (IP:67676-1-Ig, 4ug; Detection:67676-1-Ig 1:2000) with HepG2 cells lysate 1640 ug. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

PCK2 Monoclonal antibody, PBS Only

PCK2 Monoclonal Antibody for WB, IHC, IP, ELISA
Cat No. 67676-1-PBS

产品说明书

CloneNo. 1B3F1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IP, ELISA

别名

Phosphoenolpyruvate carboxykinase 2, mitochondrial, PEPCK-M, PEPCK2, mtPCK2, EC:4.1.1.32

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PCK2 Ready-To-Use IHC Kit. PCK2 primary antibody included.

产品信息

67676-1-PBS targets PCK2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 PCK2 fusion protein Ag7021 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoenolpyruvate carboxykinase 2 (mitochondrial)
别名 Phosphoenolpyruvate carboxykinase 2, mitochondrial, PEPCK-M, PEPCK2, mtPCK2, EC:4.1.1.32
计算分子量 71 kDa
观测分子量68 kDa
GenBank蛋白编号BC001454
基因名称 PCK2
Gene ID (NCBI) 5106
RRIDAB_2882871
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16822
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis. Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa.