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PCNP Polyclonal antibody

PCNP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11180-2-AP

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Synonyms

PEST-containing nuclear protein, PEST proteolytic signal-containing nuclear protein



经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11180-2-AP targets PCNP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen PCNP fusion protein Ag1663 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PEST proteolytic signal containing nuclear protein
Synonyms PEST-containing nuclear protein, PEST proteolytic signal-containing nuclear protein
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 24-28 kDa
GenBank Accession NumberBC013916
Gene Symbol PCNP
Gene ID (NCBI) 57092
RRIDAB_2160652
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WW12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCNP can be detected as 24-28 kDa after posttranslational modification.

实验方案

Product Specific Protocols
WB protocol for PCNP antibody 11180-2-APDownload protocol
IHC protocol for PCNP antibody 11180-2-APDownload protocol
IF protocol for PCNP antibody 11180-2-APDownload protocol
IP protocol for PCNP antibody 11180-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Mol Med

PCNP promotes ovarian cancer progression by accelerating β-catenin nuclear accumulation and triggering EMT transition.

Authors - Pengzhen Dong
humanWB,IHC,IF

BMC Cancer

PEST-containing nuclear protein mediates the proliferation, migration, and invasion of human neuroblastoma cells through MAPK and PI3K/AKT/mTOR signaling pathways.

Authors - Dong-Dong Wu
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