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PCNP Polyclonal antibody

PCNP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29305-1-AP

Print datasheet

Synonyms

DKFZp781I24156, PCNP

验证数据展示

  • WB analysis using 29305-1-AP

    WB analysis using 29305-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29305-1-AP (PCNP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 29305-1-AP

    IHC staining of human prostate cancer using 29305-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29305-1-AP (PCNP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA2780 cells, Jurkat cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29305-1-AP targets PCNP in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen PCNP fusion protein Ag30951 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PEST proteolytic signal containing nuclear protein
Synonyms DKFZp781I24156, PCNP
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 24-28 kDa
GenBank Accession NumberBC013916
Gene Symbol PCNP
Gene ID (NCBI) 57092
RRIDAB_2918277
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WW12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PCNP antibody 29305-1-APDownload protocol
IHC protocol for PCNP antibody 29305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols