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PCP2 Polyclonal antibody

PCP2 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat No : 13774-1-AP

Print datasheet

Synonyms

Purkinje cell protein 2, L7, GPSM4

验证数据展示

  • IHC staining of mouse cerebellum using 13774-1-AP

    IHC staining of mouse cerebellum using 13774-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13774-1-AP (PCP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 13774-1-AP

    IHC staining of mouse cerebellum using 13774-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13774-1-AP (PCP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 13774-1-AP

    IF Staining of mouse cerebellum using 13774-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using PCP2 antibody (13774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse cerebellum using 13774-1-AP

    IF Staining of mouse cerebellum using 13774-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using PCP2 antibody (13774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse cerebellum tissue, mouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13774-1-AP targets PCP2 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PCP2 fusion protein Ag4759 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Purkinje cell protein 2
Synonyms Purkinje cell protein 2, L7, GPSM4
Calculated Molecular Weight 136 aa, 15 kDa
GenBank Accession NumberBC038715
Gene Symbol PCP2
Gene ID (NCBI) 126006
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IVA1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pcp2 is uniquely expressed in cerebellar Purkinje cells and in retinal bipolar neurons, and it may function as a cell-type specific modulator for G protein-mediated cell signaling.

实验方案

Product Specific Protocols
IHC protocol for PCP2 antibody 13774-1-APDownload protocol
IF protocol for PCP2 antibody 13774-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols