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验证数据展示

  • IF Staining of Caco-2 using 83093-2-RR

    IF Staining of Caco-2 using 83093-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PCP4L1 antibody (83093-2-RR, Clone: 230203G12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of Caco-2 using 83093-2-RR

    FC experiment of Caco-2 using 83093-2-RR

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug Anti-Human PCP4L1 (83093-2-RR, Clone:230203G12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

PCP4L1 Recombinant antibody

PCP4L1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83093-2-RR

产品说明书

CloneNo. 230203G12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Purkinje cell protein 4-like protein 1, Purkinje cell protein 4 like 1, PCP4-like protein 1, PCP4 like protein 1, IQM1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inCaco-2 cells
Positive FC (Intra) detected inCaco-2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83093-2-RR targets PCP4L1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 PCP4L1 fusion protein Ag31230 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Purkinje cell protein 4 like 1
别名 Purkinje cell protein 4-like protein 1, Purkinje cell protein 4 like 1, PCP4-like protein 1, PCP4 like protein 1, IQM1
计算分子量 68 aa, 7 kDa
GenBank蛋白编号BC028905
基因名称 PCP4L1
Gene ID (NCBI) 654790
RRIDAB_3670807
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDA6NKN8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCP4L1 (Purkinje cell protein 4-like 1) is a small neuronal IQ motif protein closely related to the calmodulin-binding protein Pcp4/PEP-19 (PMID: 22458599).

实验方案

Product Specific Protocols
IF protocol for PCP4L1 antibody 83093-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols