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PCSK1 Polyclonal antibody

PCSK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28219-1-AP

Print datasheet

Synonyms

BMIQ12, NEC 1, NEC1, Neuroendocrine convertase 1, PC1, PC3, PCSK1, Prohormone convertase 1, Proprotein convertase 1, SPC3

验证数据展示

  • WB analysis using 28219-1-AP

    WB analysis using 28219-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28219-1-AP (PCSK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 28219-1-AP

    IHC staining of human pancreas using 28219-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas using 28219-1-AP

    IHC staining of human pancreas using 28219-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells
Positive IHC detected inhuman pancreas tissue, mouse pancreas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28219-1-AP targets PCSK1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity Human, Mouse, rat
Cited Reactivityhuman, mouse
Immunogen PCSK1 fusion protein Ag28203 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proprotein convertase subtilisin/kexin type 1
Synonyms BMIQ12, NEC 1, NEC1, Neuroendocrine convertase 1, PC1, PC3, PCSK1, Prohormone convertase 1, Proprotein convertase 1, SPC3
Calculated Molecular Weight 753 aa, 84 kDa
Observed Molecular Weight66 kDa
GenBank Accession NumberBC130295
Gene Symbol PCSK1
Gene ID (NCBI) 5122
RRIDAB_2881093
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29120
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PC1/3, encoded by the gene PCSK1, is synthesized in the endoplasmic reticulum (ER) as an inactive 94 kDa zymogen, where it is rapidly cleaved into a partially active 87-kDa precursor. When this protein reaches the secretory granules, the maturation process continues to generate a 74 kDa species and ends with further cleavage to a fully active, although enzymatically unstable, 66 kDa protein.

实验方案

Product Specific Protocols
WB protocol for PCSK1 antibody 28219-1-APDownload protocol
IHC protocol for PCSK1 antibody 28219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

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humanIHC

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Data-independent acquisition combined with liquid chromatography mass spectrometry technique to detect prognostic protein markers in type I gastric neuroendocrine neoplasm

Authors - Meng Zhang
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