PCSK2 Polyclonal antibody
PCSK2 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
10553-1-AP targets PCSK2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | PCSK2 fusion protein Ag0852 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | proprotein convertase subtilisin/kexin type 2 |
| Synonyms | KEX2 like endoprotease 2, NEC 2, NEC2, Neuroendocrine convertase 2, PC2, PCSK2, Prohormone convertase 2, Proprotein convertase 2, SPC2 |
| Calculated Molecular Weight | 70.5 kDa |
| Observed Molecular Weight | 65-75 kDa |
| GenBank Accession Number | BC005815 |
| Gene Symbol | PCSK2 |
| Gene ID (NCBI) | 5126 |
| RRID | AB_2299124 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16519 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PCSK2(Prohormone convertase 2) is also named as NEC2(neuroendocrine convertase 2), PC2 and belongs to the peptidase S8 family. It is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. PCSK2 is responsible for the release of glucagon from proglucagon in pancreatic A cells. This protein has 3 isoforms produced by alternative splicing. PCSK2 is biosynthesized as a 74-kDa inactive precursor which is matured to a 64-kDa active enzyme by autocatalytic removal of its prodomain (PMID: 8397508, 21092739)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCSK2 antibody 10553-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells. | ||
Function (Oxf) ER Redox Homeostasis Regulates Proinsulin Trafficking and Insulin Granule Formation in the Pancreatic Islet β-Cell | ||
iScience Insulin receptor isoform B is required for efficient proinsulin processing in pancreatic β cells |
