验证数据展示
经过测试的应用
| Positive IHC detected in | human kidney tissue, human brain tissue, human skin tissue, human heart tissue, human testis tissue, human spleen tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
16470-1-AP targets PCSK5 in IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, rat |
| 免疫原 | PCSK5 fusion protein Ag9552 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proprotein convertase subtilisin/kexin type 5 |
| 别名 | hPC6, PC5, PC6, PC6A, PCSK5, Proprotein convertase 5, Proprotein convertase 6, SPC6 |
| 计算分子量 | 102 kDa, 207 kDa |
| 观测分子量 | 210 kDa |
| GenBank蛋白编号 | BC012064 |
| 基因名称 | PCSK5 |
| Gene ID (NCBI) | 5125 |
| RRID | AB_2283381 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92824 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PC5 ,a mammalian kex2-like processing endoprotease, mediates posttranslational endoproteolytic processing for several integrin alpha subunits.It has been identified in rat and mouse endocrine and neuroendocrine tissues,and is encoded by multiple mRNAs that generate two splice variant isoforms referred to as PC5A (102KDa) and PC5B (207KDa) .PC5A is smaller than PC5B and it can be processed into two forms (117 kDa and 65 kDa) in neuronal cells(PMID:11457520).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PCSK5 antibody 16470-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Med Rep MicroRNA‑338‑3p regulates age‑associated osteoporosis via targeting PCSK5.
| ||
Biochem Biophys Rep Secreted factors from adipose tissue-derived mesenchymal stem cells suppress oxygen/glucose deprivation-induced cardiomyocyte cell death via furin/PCSK-like enzyme activity. | ||
Mol Cell Biochem PCSK5 downregulation promotes the inhibitory effect of andrographolide on glioblastoma through regulating STAT3 | ||
Circ Heart Fail Overexpression of ATP5F1A in Cardiomyocytes Promotes Cardiac Reverse Remodeling |
