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验证数据展示

  • IF Staining of HepG2 using 83004-4-RR

    IF Staining of HepG2 using 83004-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • FC experiment of HeLa using 83004-4-RR

    FC experiment of HeLa using 83004-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PCSK9 Recombinant antibody (83004-4-RR, Clone:230536E4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse liver using 83004-4-RR

    IF Staining of mouse liver using 83004-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 83004-4-RR

    IF Staining of mouse liver using 83004-4-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83004-4-RR

    Affinity and Kinetic Characterization of 83004-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83004-4-RR against Mouse PCSK9 were performed. The affinity constant is 384 nM.

PCSK9 Recombinant antibody

PCSK9 Uni-rAbTM Recombinant Antibody for IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 83004-4-RR

产品说明书

CloneNo. 230536E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, IF-P, FC (Intra), ELISA

别名

Narc1, HCHOLA3, FH3, AI747682, AI415265

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse liver tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83004-4-RR targets PCSK9 in IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 proprotein convertase subtilisin/kexin type 9
别名 Narc1, HCHOLA3, FH3, AI747682, AI415265
计算分子量75KD
GenBank蛋白编号NM_153565.2
基因名称 Pcsk9
Gene ID (NCBI) 100102
RRIDAB_3670749
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ80W65
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCSK9 (Proprotein convertase subtilisin/kexin type 9) is the ninth member of the proprotein convertase family that regulates lipoprotein homeostasis and altered PCSK9 expression was reportedly associated with tumor development and progression. PCSK9 is primarily synthesized and expressed in the liver but also in many other tissues, such as the intestinal tract, kidney, brain, heart, and blood vessels (PMID: 36242053).

实验方案

Product Specific Protocols
IF protocol for PCSK9 antibody 83004-4-RRDownload protocol
FC protocol for PCSK9 antibody 83004-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols