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PCSK9 Recombinant antibody

PCSK9 Uni-rAbTM Recombinant Antibody for IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230536E4

Cat no : 83004-4-RR

Print datasheet

Synonyms

Narc1, HCHOLA3, FH3, AI747682, AI415265

验证数据展示

  • IF Staining of HepG2 using 83004-4-RR

    IF Staining of HepG2 using 83004-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • FC experiment of HeLa using 83004-4-RR

    FC experiment of HeLa using 83004-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PCSK9 Recombinant antibody (83004-4-RR, Clone:230536E4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse liver using 83004-4-RR

    IF Staining of mouse liver using 83004-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 83004-4-RR

    IF Staining of mouse liver using 83004-4-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83004-4-RR

    Affinity and Kinetic Characterization of 83004-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83004-4-RR against Mouse PCSK9 were performed. The affinity constant is 384 nM.

经过测试的应用

Positive IF-P detected inmouse liver tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83004-4-RR targets PCSK9 in IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with mouse samples.

Tested Applications IF/ICC, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name proprotein convertase subtilisin/kexin type 9
Synonyms Narc1, HCHOLA3, FH3, AI747682, AI415265
Calculated Molecular Weight75KD
GenBank Accession NumberNM_153565.2
Gene Symbol Pcsk9
Gene ID (NCBI) 100102
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ80W65
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCSK9 (Proprotein convertase subtilisin/kexin type 9) is the ninth member of the proprotein convertase family that regulates lipoprotein homeostasis and altered PCSK9 expression was reportedly associated with tumor development and progression. PCSK9 is primarily synthesized and expressed in the liver but also in many other tissues, such as the intestinal tract, kidney, brain, heart, and blood vessels (PMID: 36242053).

实验方案

Product Specific Protocols
IF protocol for PCSK9 antibody 83004-4-RRDownload protocol
FC protocol for PCSK9 antibody 83004-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols