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PCTAIRE3 Polyclonal antibody

PCTAIRE3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12207-1-AP

Print datasheet

Synonyms

PCTK3, PCTAIRE-motif protein kinase 3, PCTAIRE, EC:2.7.11.22, Cyclin-dependent kinase 18

验证数据展示

  • IF Staining of HEK-293 using 12207-1-AP

    IF Staining of HEK-293 using 12207-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 12207-1-AP (PCTAIRE3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of HEK-293 using 12207-1-AP

    WB analysis of HEK-293 using 12207-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12207-1-AP (PCTAIRE3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 12207-1-AP

    IHC staining of human ovary cancer using 12207-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12207-1-AP (PCTAIRE3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCTAIRE3 antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy PCTAIRE3 Ready-To-Use IHC Kit. PCTAIRE3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12207-1-AP targets PCTAIRE3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PCTAIRE3 fusion protein Ag2851 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PCTAIRE protein kinase 3
Synonyms PCTK3, PCTAIRE-motif protein kinase 3, PCTAIRE, EC:2.7.11.22, Cyclin-dependent kinase 18
Calculated Molecular Weight 472 aa, 54 kDa
Observed Molecular Weight 54 kDa, 58 kDa
GenBank Accession NumberBC011526
Gene Symbol PCTAIRE3
Gene ID (NCBI) 5129
RRIDAB_906375
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07002
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCTAIRE3 (PCTAIRE-motif protein kinase 3), also named CDK18 or PCTK3, is a member of PCTAIRE subfamily. The PCTAIRE kinase (PCTK) subfamily of CDKs includes three members, PCTK1/CDK16, PCTK2/CDK17, and PCTK3/CDK18, which contain the PCTAIRE sequence instead of the PSTAIRE sequence (PMID: 24831015). PCTK3 has three isoforms with the molecular mass of 54-58kD.

实验方案

Product Specific Protocols
WB protocol for PCTAIRE3 antibody 12207-1-APDownload protocol
IHC protocol for PCTAIRE3 antibody 12207-1-APDownload protocol
IF protocol for PCTAIRE3 antibody 12207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols