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  • KD/KO Validated

PCYT2 Polyclonal antibody

PCYT2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 14827-1-AP

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Synonyms

Ethanolamine-phosphate cytidylyltransferase, Ethanolamine phosphate cytidylyltransferase, EC:2.7.7.14



经过测试的应用

Positive WB detected inhuman liver tissue, HepG2 cells, MCF-7 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14827-1-AP targets PCYT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen PCYT2 fusion protein Ag6616 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphate cytidylyltransferase 2, ethanolamine
Synonyms Ethanolamine-phosphate cytidylyltransferase, Ethanolamine phosphate cytidylyltransferase, EC:2.7.7.14
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC000351
Gene Symbol PCYT2
Gene ID (NCBI) 5833
RRIDAB_2159298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99447
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ethanolamine-phosphate cytidylyltransferase (PCYT2) catalyzes the second step in the synthesis of phosphatidylethanolamine (PE) from ethanolamine via the CDP-ethanolamine pathway (PMID:9083101, PMID:31637422). Phosphatidylethanolamine is a dominant inner-leaflet phospholipid in cell membranes, where it plays a role in membrane function by structurally stabilizing membrane-anchored proteins, and participates in important cellular processes such as cell division, cell fusion, blood coagulation, and apoptosis (PMID:9083101).

实验方案

Product Specific Protocols
WB protocol for PCYT2 antibody 14827-1-APDownload protocol
IF protocol for PCYT2 antibody 14827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

Metabolic control of TFH cells and humoral immunity by phosphatidylethanolamine.

Authors - Guotong Fu
  • KO Validated
humanIHC

Int J Mol Sci

Cancer Malignancy Is Correlated with Upregulation of PCYT2-Mediated Glycerol Phosphate Modification of α-Dystroglycan.

Authors - Fumiko Umezawa
human,mouseIF,WB

Int J Biol Macromol

p75NTR enhances cognitive dysfunction in a mouse Alzheimer's disease model by inhibiting microRNA-210-3p-mediated PCYT2 through activation of NF-κB

Authors - Zhongliang Wei
  • KD Validated
humanWB

Evid Based Complement Alternat Med

Metabolomic Study on Nude Mice Models of Gastric Cancer Treated with Modified Si Jun Zi Tang via HILIC UHPLC-Q-TOF/MS Analysis.

Authors - Shanshan Nie