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PCYT2 Monoclonal antibody, PBS Only

PCYT2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F7G1

Cat no : 68355-1-PBS

Print datasheet

Synonyms

ET, PCYT2

验证数据展示

  • WB analysis using 68355-1-Ig (same clone as 68355-1-PBS)

    WB analysis using 68355-1-Ig (same clone as 68355-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68355-1-Ig (PCYT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68355-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68355-1-Ig (same clone as 68355-1-PBS)

    IF Staining of HepG2 using 68355-1-Ig (same clone as 68355-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCYT2 antibody (68355-1-Ig, Clone: 2F7G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68355-1-PBS in a different storage buffer formulation.

产品信息

68355-1-PBS targets PCYT2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen PCYT2 fusion protein Ag6604 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name phosphate cytidylyltransferase 2, ethanolamine
Synonyms ET, PCYT2
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC000351
Gene Symbol PCYT2
Gene ID (NCBI) 5833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99447
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ethanolamine-phosphate cytidylyltransferase (PCYT2) catalyzes the second step in the synthesis of phosphatidylethanolamine (PE) from ethanolamine via the CDP-ethanolamine pathway (PMID:9083101, PMID:31637422). Phosphatidylethanolamine is a dominant inner-leaflet phospholipid in cell membranes, where it plays a role in membrane function by structurally stabilizing membrane-anchored proteins, and participates in important cellular processes such as cell division, cell fusion, blood coagulation, and apoptosis (PMID:9083101).